Up-Streaming Process for Glucose Oxidase by Thermophilic Penicillium sp. in Shake Flask
نویسندگان
چکیده
The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp. in a 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. The effect of submerged fermentation in shaking and static conditions, different carbon sources and incubation period on the production of extra-cellular glucose oxidase was studied. A maximum yield (0.325 U/mL) of extracellular glucose oxidase was obtained after 72 h of incubation at 45 °C using submerged fermentation under shaking utilizing sucrose as a carbon source.
منابع مشابه
L-isoleucine Effects on Yield and Productivity of a Batch Fermentation Process for Mycophenolic Acid Production by Penicillium brevicompactum
Mycophenolic acid (MPA) is an antibiotic and immunosuppressive agent has been produced as a secondary metabolite of some fungi species such as Penicillium. In the present work, in the first step, mycophenolic acid production was performed in a fermentation process by Penicillium brevicompactum MUCL 19011 in shake flask using a base medium. Obtained results demonstrated the maximum MPA productio...
متن کاملEnhanced Mycophenolic Acid Production by Penicillium brevicompactum with Enzymatically Hydrolyzed Casein
Mycophenolic acid (MPA) is a secondary metabolite produced by Penicillium brevicompactum, which has antibiotic and immunosuppressive properties. In this study, the first, mycophenolic acid was produced in a fermentation process by Penicillium brevicompactum MUCL 19011 in shake flask using a base medium. The maximum MPA production, product yield and productivity of process were 1.379 g/L, 18.6 m...
متن کاملTransformation and expression of Penicillium funicolusum glucose oxidase gene in yeast
Glucose oxidase is an important enzyme hydrolyzing for its hydrolyzing activity on glucos. It possesses and has a wide board of applications in different industries such as bakery, pharmaceutical, plant pathology and biosensors. In this study, yeast (Saccharomyces cerevisiae) was transformed successfully by the glucose oxidase gene (gox) obtained from Penicillium funicolusum. The secreted gluco...
متن کاملOptimization of Inoculum Volume, Fermentation Medium and Aeration Rate for the Production of Glucose Oxidase by Uv Mutant Strain of Aspergillus Niger An-14
In this study the effect of inoculum size, volume of fermentation medium and aeration rate was analyzed for UV mutated strain of Aspergillus niger AN-14 regarding enhanced production of glucose oxidase. UV treated 10 variants showing positive variations from parent strain were isolated and analyzed for glucose oxidase production using shake flask fermentation. Mutant strain AN-14 gave maximum p...
متن کاملMycophenolic Acid Production: Investigation of the Effects of Enzymatically Hydrolyzed Casein Concentration on Product Yield and Productivity
Mycophenolic acid (MPA) is an antibiotic and immunosuppressive drug produced by Penicillium strains as a secondary extracellular metabolite. In this work, the effect of some various concentrations of enzymatically hydrolyzed casein on mycophenolic acid yield and process productivity was studied. The first, a base medium was applied for mycophenolic acid production by Penicillium brevicompactum ...
متن کامل